TY - JOUR ID - 74129 TI - Evaluation of Flow Cytometry and Kleihauer Techniques for Quantification of Fetomaternal Hemorrhage: A Prospective Cohort Study in Southwestern Iran JO - Hospital Practices and Research JA - HPR LA - en SN - 2476-390X AU - Keshavarz, Zeinab AU - Moezzi, Leili AU - Ranjbaran, Reza AU - Behzad-Behbahani, Abbas AU - Abdullahi, Masooma AU - Mahmoodi, Mahdokht AU - Sharifzadeh, Sedigheh AD - Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran Y1 - 2018 PY - 2018 VL - 3 IS - 4 SP - 118 EP - 122 KW - Fetomaternal Transfusion KW - Rho (D) Immune Globulin KW - Flow Cytometry KW - Pregnancy KW - Fetus KW - Rh Blood-Group System DO - 10.15171/hpr.2018.26 N2 - Background: Quantification of fetal red blood cells (RBCs) in maternal blood is of great importance to calculate appropriate dose of post-deliver anti D immunoglobulin in a rhesus D (RhD)-negative woman. Objective: The aim of this study is to evaluate a direct immunofluorescence flow cytometry technique in artificial and clinical samples and compared it to the Kleihauer-Betke test (KBT). Methods: This study was a prospective cohort design. Blood samples from 26 pregnant women who gave birth to RhD positive babies were tested using direct immunofluorescence flow cytometry and KBT techniques to determine the amount of FMH in the maternal circulation. The zone of D-positive cells was identified employing artificial samples including 0.3%, 0.6%, 1%, 1.5%, 2%, 5%, 10%, and 50% of D-positive fetal cells in D-negative maternal cells. Results: Analysis of 26 clinical samples for FMH showed consistent quantification with the flow cytometry and Kleihauer techniques. Although a good correlation was found between the KBT and flow cytometry results, in artificial samples containing more than 2% of fetal RhD positive cells, the flow cytometry results were closer to theoretical percentages. In a patient with FMH >4 mL, the FMH and consequently the required vial of Ig were overestimated using KBT. Conclusion: Most of the FMH calculated could have been neutralized by doses less than 625 IU, whereas the routine dose in Iran is more than double that amount (1500 IU). This achievement demonstrates that adjusting between the RhD immune globulin (RhDIg) dose and FMH size is inevitable. UR - https://www.jhpr.ir/article_74129.html L1 - https://www.jhpr.ir/article_74129_c865fa028422e59c971312f47dd83228.pdf ER -