Hospital Practices and Research

Frequency of blaSHV and blaTEM Genes in Clinical Isolates of Enterobacter, along with the Determination of Antibiotic and Probiotic Resistance Patterns

Document Type : Original Article

Authors

Fariba Ghaderi 1
Zahra Hojjati Bonab 1
Somayyeh Taghizadeh 2

1 Department of Microbiology, Bon.C., Islamic Azad University, Bonab, Iran

2 Department of Biology Education, Farhangian University, Tehran, Iran

https://doi.org/10.30491/hpr.2025.484234.1455
Abstract
Background: Urinary tract infections are among the most prevalent human infections, primarily caused by Enterobacteriaceae. Currently, a significant number of Enterobacteriaceae produce extended-spectrum beta-lactamases (ESBLs), rendering them resistant to beta-lactam antibiotics and resulting in treatment failures.
Objectives: The aim of this study was to determine the frequency of blaSHV and blaTEM genes in Enterobacter isolates from clinical samples at Amir al-Momenin Hospital in Maragheh city in 2023, as well as to assess their antibiotic and probiotic resistance patterns.
Methods: One hundred urine samples from patients with urinary tract infections hospitalized at Amir Al-Momenin Hospital were included in the study. ESBL-producing bacteria were identified using the agar disk diffusion method according to CLSI criteria, employing 30 µg ceftazidime and cefotaxime antibiotic disks, both with and without clavulanic acid. PCR was utilized to amplify the genes for examining the frequency of blaTEM and blaSHV genes. Electrophoresis of the samples was conducted on a 1% agarose gel.
Results: Of the 21 samples, the ESBL index was negative in 7 samples, resulting in a frequency of 33.33%, while it was positive in 14 samples, with a frequency of 66.67%. The frequency of the blaTEM gene in positive bacterial samples was 95.23%, and the frequency of the blaSHV gene was 92.52%.
Conclusion: The disk diffusion test on antibiotic-sensitive samples found that the highest average growth inhibition zone was associated with the CTC antibiotic, while the lowest average growth inhibition zone was related to the NA antibiotic.

Keywords

Disk Diffusion
Enterobacteriaceae
ESBL
blaTEM
blaSHV
  1. Elsherief MF, Mousa MM, El-Galil HA, El-Bahy EF. Enterobacteriaceae Associated with Farm Fish and Retailed Ones. Alex J Vet Sci. 2014;42(1). doi:10.54 55/ajvs.153299
  2. Patel KK, Patel S. Enterobacter: an emerging nosocomial infection. Int J Appl Eng Res. 2016;2(11):532-8. doi:10.1016/0195-6701(88)90098-9
  3. Mezzatesta ML, Gona F, Stefani S. Enterobacter cloacae complex: clinical impact and emerging antibiotic resistance. Future Microbiol. 2012;7(7):887-902. doi:10.2217/fmb.12.61
  4. Izdebski R, Baraniak A, Herda M, Fiett J, Bonten MJ, Carmeli Y, et al. MLST reveals potentially high-risk international clones of Enterobacter cloacae. J Antimicrob Chemother. 2015;70(1):48-56. doi:10.10 93/jac/dku359
  5. Potron A, Poirel L, Rondinaud E, Nordmann P. Intercontinental spread of OXA-48 beta-lactamase-producing Enterobacteriaceae over a 11-year period, 2001 to 2011. Euro Surveill. 2013;18(31):20549. doi:10.2807/1560-7917.es2013.18.31.20549
  6. Davin-Regli A, Pag s JM. Enterobacter aerogenes and Enterobacter cloacae; versatile bacterial pathogens confronting antibiotic treatment. Front Microbiol. 2015;6:392. doi:10.3389/fmicb.2015.00392
  7. Iversen C, Waddington M, Farmer JJ 3rd, Forsythe SJ. The biochemical differentiation of Enterobacter sakazakii BMC Microbiol. 2006;6:94. doi:10.1186/1471-2180-6-94
  8. Wolf DC, Giannella RA. Antibiotic therapy for bacterial enterocolitis: a comprehensive review. Am J Gastroenterol. 1993;88(10):1667-83.
  9. Ferreira RL, da Silva BCM, Rezende GS, Nakamura-Silva R, Pitondo-Silva A, Campanini EB, et al. High Prevalence of Multidrug-Resistant Klebsiella pneumoniae Harboring Several Virulence and β-Lactamase Encoding Genes in a Brazilian Intensive Care Unit. Front Microbiol. 2019;9:3198. doi:10.3389/fmicb.2018. 03198
  10. Ponce de León-Rosales S, Arredondo-Hern ndez R, López-Vidal Y. Resistance to antibiotic: A serious global problem. Gac Med Mex. 2015;151(5):681-9.
  11. T th AG, Csabai I, Judge MF, Mar ti G, Becsei , Spis k S, Solymosi N. Mobile Antimicrobial Resistance Genes in Probiotics. Antibiotics. 2021; 10(11):1287. doi:10.3390/antibiotics10111287
  12. Corr SC, Hill C, Gahan CG. Understanding the mechanisms by which probiotics inhibit gastrointestinal pathogens. Adv Food Nutr Res. 2009; 56:1-15. doi:10.1016/S1043-4526(08)00601-3
  13. Gambarotto K, Ploy MC, Turlure P, Gr laud C, Martin C, Bordessoule D, et al. Prevalence of vancomycin-resistant enterococci in fecal samples from hospitalized patients and nonhospitalized controls in a cattle-rearing area of France. J Clin Microbiol. 2000;38(2):620-4. doi:10.1128/JCM.38.2.620-624.2 000
  14. Gikas A, Christidou A, Scoulica E, Nikolaidis P, Skoutelis A, Levidiotou S, et al. Epidemiology and molecular analysis of intestinal colonization by vancomycin-resistant enterococci in greek hospitals. J Clin Microbiol. 2005;43(11):5796-9. doi:10.1128/ JCM.43.11.5796-5799.2005
  15. Philippon A, Slama P, D ny P, Labia R. A Structure-Based Classification of Class A β-Lactamases, a Broadly Diverse Family of Enzymes. Clin Microbiol Rev. 2016;29(1):29-57. doi:10.1128/CMR.00019-15
  16. Rupp ME, Fey PD. Extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae: considerations for diagnosis, prevention and drug treatment. Drugs. 2003;63(4):353-65. doi:10.2165/00003495-2003630 40-00002
  17. Munita JM, Arias CA. Mechanisms of antibiotic resistance. Virulence mechanisms of bacterial pathogens. 2016: 481-511. doi:10.1128/microbiolspec.VMBF-0016-2015
  18. Carcione D, Siracusa C, Sulejmani A, Leoni V, Intra J. Old and new beta-lactamase inhibitors: molecular structure, mechanism of action, and clinical use. Antibiotics. 2021;10(8):995. doi:10.3390/antibiotics 10080995
  19. Chokshi A, Sifri Z, Cennimo D, Horng H. Global contributors to antibiotic resistance. J Glob Infect Dis. 2019;11(1):36-42. doi:10.4103/jgid.jgid_110_18
  20. Kim SY, Jang MS, Kim J. Impact of Third-Generation Cephalosporin Resistance on Recurrence in Children with Febrile Urinary Tract Infections. J Pers Med. 2022;12(5):773. doi:10.3390/jpm12050773
  21. Zandi H, Tabatabaei SM, Ehsani F, Zarch MB, Doosthosseini S. Frequency of Extended-Spectrum Beta-lactamases (ESBLs) in strains of Klebsiella and  coliisolated from patients hospitalized in Yazd. Electron Physician. 2017;9(2):3810-3815. doi:10.19 082/3810
  22. Vachvanichsanong P, McNeil EB, Dissaneewate P. Extended-spectrum beta-lactamase Escherichia coli and Klebsiella pneumoniae urinary tract infections. Epidemiol Infect. 2021;149:e12. doi:10.1017/S09 50268820003015
  23. Bajpai T, Pandey M, Varma M, Bhatambare GS. Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital. Avicenna J Med. 2017;7(01):12-6. doi:10.4103/2231-0770.197508

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