Hospital Practices and Research

Biological Synthesis of Nanoparticles and Evaluation of Their Synergistic Antimicrobial Effects with Algae Extract Against Multidrug-Resistant Bacteria: A Policy Summary and Applied Approach

Articles in Press

Authors

Fatemeh Elahi 1
Hamid Babavalian 2

1 Student Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran

2 Applied Biotechnology Research Center, Institute for New Health Technologies, Baqiyatallah University of Medical Sciences, Tehran, Iran

10.30491/hpr.2026.256769
Abstract
Selenium nanoparticles (SeNPs) were biosynthesized using the bacterium Kocuria, isolated from the Hera mangrove ecosystem in Iran. After confirming their structural and chemical properties, the SeNPs were tested—both alone and in combination with Chlorella vulgaris crude extract against multidrug-resistant bacteria. The combination effectively inhibited the growth of Escherichia coli, Staphylococcus epidermidis, and Staphylococcus saprophyticus, but did not affect A. baumannii. Cytotoxicity tests showed that while SeNPs and Chlorella vulgaris individually had low toxicity, their combination increased cytotoxic effects. Overall, the SeNPs- Chlorella vulgaris combination shows promise as a novel approach to combat resistant bacteria, though further optimization and safety evaluations are needed.

Keywords

Selenium Nanoparticles
Kocuria
Chlorella vulgaris
Antimicrobial Activity
Multidrug-Resistance
Bioremediation
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2.    Babavalian H, Shakeri F, Safarpour A, Hassankhani I, Nasrollahzadeh Z, Amoozegar MA. Biosynthesis and Characterization of Selenium Nanoparticles by Kocuria Strain Bm3: Evaluating Their Synergistic Antimicrobial Activity with Chlorella vulgaris Crude Extract Against Multidrug-Resistant Bacteria. Iran J Pharm Res. 2025;24(1):e164350. doi:10.5812/ijpr-164350
3.    Eladl SN, Elnabawy AM, Eltanahy EG. Recent biotechnological applications of value-added bioactive compounds from microalgae and seaweeds. Bot Stud. 2024;65(1):28. doi:10.1186/s40529-024-00434-y 
4.    Mohammad AM, Radwan OA, Khalel AF, Al-Alfard AA, Mekky A, Seyam EA. Dual efficacy of Chlorella vulgaris extract and neem seed oil as antifungal agents and plant growth promoters against Fusarium oxysporum in maize. J Radiat Res Appl Sci. 2026; 19(1):102093. doi:10.1016/j.jrras.2025.102093 
Hospital Practices and Research

Articles in Press, Accepted Manuscript
Available Online from 10 November 2026

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