Hospital Practices and Research

Effects of Different Times of Glutaraldehyde 2% on Bacillus subtilis Spores (In Vitro)

Document Type : Original Article

Authors

Eshagh Lasemi 1
Mohammad Hossain Kalantar Motamedi 1, 2
Fina Navi 1
Maryam Rezae 3
Niousha Homay Nikfar 4
Zahra Danial 2, 5
Roojan Azizpour 1

1 Craniomaxillofacial Research Center, Tehran Dental Branch, Islamic Azad University, Tehran, Iran

2 Trauma Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

3 Department of Microbiology, Dental Branch, Islamic Azad University, Tehran, Iran

4 Private Practice

5 Faculty of Psychology & Educational Sciences, Allameh Tabataba’i University, Tehran, Iran

https://doi.org/10.15171/hpr.2017.28
Abstract
Background: Due to the importance of disinfectant and sterilization of dental instruments, in total, 14%–28% of dentists, 13% of assistants, and 17% of healthcare workers (HCWs) have been subjected to the hepatitis B virus (HBV), and more than 200 healthcare providers (HCPs) pass away annually in the United States from HBV infection catched from their work place.
Objective: This study examined the effects of glutaraldehyde 2% on Bacillus subtilis spores in the Surgery and Microbiology Department of the Dental Branch of Islamic Azad University.
Methods: This experimental research evaluated a total of 58 samples, one called first evidence (pure glutaraldehyde not exposed to spore suspension), one called second evidence (spore suspension not exposed to glutaraldehyde), and 40 samples including a suspension with a normal turbidity of 1×108 CFU/mL according to 0.5 McFarland with exposure to glutaraldehyde 2%. Experiments were done in time intervals of 10, 15, 20, 25, 30, 40, and 60 minutes with 8 repeats. In all times, a B. subtilis spore suspension was used as evidence and also as a case.
Results: This research was done on 58 samples. The results showed that in the 10th minute there were 102 colonies, 18.6 ± 3.4 in the 15th minute, 6.2 ± 1.4 in the 20th minute, 2.1 ± 0.8 in the 25th minute, and no colonies after 30 minutes. In an overall observation, it was seen that there were more colonies in the first 10 minutes, and from 15 to 20 minutes, this amount significantly decreased; after 30 minutes in each 8 repeats, the growth of colonies had stopped completely, while in the evidence samples, B. subtilis spores grew.
Conclusion: It seems that the density of 2% glutaraldehyde in 30 minutes time was enough to destroy the spores of B. subtilis.

Keywords

Glutaraldehyde
Bacillus Subtilis Spores
Disinfection
  1. Farahani M, Sanaei AS. Infection control at dental office. Tehran: Baraye Farad; 2008:13-45. [Persian].
  2. Heymann HO, Swift EJ Jr, Ritter AV. Sturdevant’s Art and Science of Operative Dentistry. 6th ed. London: Mosby; 2012:376-81.
  3. Etesam F. Principle of Biology. Tehran: Islamic Azad Universuty, Tehran Dental Branch; 1986:1-6. [Persian].
  4. Adibfar P. Medical Microbiology. Tehran: Nor-e-Danesh Co; 2004:279-289. [Persian].
  5. Sepkowitz KA. Occupationally acquired infections in health care workers. Part II. Ann Intern Med. 1996;125(11):917-928. doi:10.7326/0003-4819-125-11-199612010-00008.
  6. Alamdari A, Behrouzi M, Hosseini SM, Sadeghi H, Azizi A, Ghafarian Shirazi HR. Comparison of two disinfectant solutions deconex 53 plus 2% and glutaraldehyde (Cidex) 2% on reduction of medical equipment contamination in the operating room of urology ward. Ann Trop Med Public Health. 2017;10(4):910-913.
  7. Coates D. Sporicidal activity of sodium dichloroisocyanurate, peroxygen and glutaraldehyde disinfectants against Bacillus subtilis. J Hosp Infect. 1996;32(4):283-294. doi:10.1016/S0195-6701(96)90039-0.
  8. Anderson TR, Slotkin TA. Maturation of the adrenal medulla--IV. Effects of morphine. Biochem Pharmacol. 1975;24(16):1469- 1474. doi:10.1016/0006-2952(75)90020-9.
  9. Omidkhoda M, Rashed R, Bagheri Z, Ghazvini K, Shafaee H. Comparison of three different sterilization and disinfection methods on orthodontic markers. J Orthod Sci. 2016;5(1):14-17. doi:10.4103/2278-0203.176653.
  10. de Almeida CMF, de Carvalho AS, Duarte DA. Evaluation of disinfection methods of orthodontic pliers. Dental Press J Orthod. 2012;17(4):105-109. doi:10.1590/S2176- 94512012000400020.
  11. Simoes LC, Lemos M, Araujo P, Pereira AM, Simoes M. The effects of glutaraldehyde on the control of single and dual biofilms of Bacillus cereus and Pseudomonas fluorescens. Biofouling. 2011;27(3):337-346. doi:10.1080/08927014.2011.575935.
  12. Retta SM, Sagripanti JL. Modeling the inactivation kinetics of bacillus spores by glutaraldehyde. Lett Appl Microbiol. 2008;46(5):568-574. doi:10.1111/j.1472-765X.2008.02358.x.
  13. Miller C, Palenic C. Infection Control and Management of Hazardous Material for Dental Team. 2nd ed. St. Louis: Mosby; 1998:3-6.
  14. da Silva FC, Kimpara ET, Mancini MN, Balducci I, Jorge AO, Koga-Ito CY. Effectiveness of six different disinfectants on removing five microbial species and effects on topographic characteristics of acrylic resin. J Prosthodont. 2008;17(8):627-633. doi:10.1111/j.1532-849X.2008.00358.x.
  15. Russell AD. Bacterial spores and chemical sporicidal agents. Clin Microbiol Rev. 1990;3(2):99-119. doi:10.1128/CMR.3.2.99.
  16. Miner N, Harris V, Cao TD, Ebron T, Lukomski N. Aldahol high-level disinfectant. Am J Infect Control. 2010;38(3):205-211. doi:10.1016/j.ajic.2009.08.009.
  17. Navi F, Dejahang S. Comparison of concentration of 2% and 6% Nanosil on Bacillus subtilis spores (in vitro) [dissertation]. Tehran: School of dentistry, Azad University of Tehran; 2014. [Persian].
Hospital Practices and Research
Volume 2, Issue 4 - Serial Number 8
Autumn 2017
Pages 118-121

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